448 The Botanical Gazette. [December, 
made by glueing a glass ring toa slide. The bottom of the 
cell had previously been moistened with water, so that a min- 
iature damp chamber was thus formed. In order to more 
certainly guard against drying, the further precaution was 
observed, of placing the cultures themselves in a large damp 
chamber. Occasionally, simple watch glass cultures were 
prepared, but these were seldom found to be useful, on ac- 
count of their great liability to contamination. 
1. Effects of different chemicals upon germination. 
_ _In carrying on the experiments in this line, one or more 
check cultures in water were always included in every series 
of cultures prepared at any one time. The results of the cul- 
tures in the various solutions were compared with those of the 
water cultures, and in every series where germination failed, 
both in water and in the other solutions, the whole experiment 
was discarded, ofcourse. The uredospores of grain rusts re a 
used in most of the experiments, particularly the uredo Of, 
Puccinia coronata Corda, since that species was the most 
abundant for the longest period in 1892, when the aps 
of these experiments were performed. In the following ta a 
I give the names of those solutions only which show the pi 
striking results, either favorable or unfavorable. The ta e 
represent only a small proportion of the cultures ace of 
The numbers in the column ‘‘strength” give the num "i ip 
parts, by weight, of the commercially prepared ee 4 
10,000 parts of distilled water, in which they were disso vot 
The first table shows results obtained with uredospore 
Puccinia Rubigo-vera (DC.) Wint.: : 
eee e 
SETS a 
al 
ee 
ay 
i 
