Mica 
ij 
456 The Botanical Gazette. [December, 
pappus spinulosus, and in the mesospores of P. Sporoboli 
rth. This process of germination may prove to be of im- 
portance in classification, and help to clear up the existing 
confusion regarding some of our western so-called LEPTO-PUC- 
CINI4 on composites. Iam not yet prepared, nor have I the 
space in this article, to make any statements, on this basis, 
concerning the systematic position of such species. 
In two other cases, those of Puccinia Malvastri Pk. and 
the Puccinia of Lygodesmia juncea, the indications were that 
catenulate sporidiola would be formed, but germination 
ceased before reaching that stage. The germination of Puc- 
cinia Sporoboli was the most remarkable. In this case, nota 
single two-celled spore germinated while the mesospores get- 
minated profusely, uniformly producing catenulate sporidiola. 
All these species, except Puccinia Sporoboli, were collected 
in October, 1892, and germinated after being kept in paper 
pockets till April, 1893. It may be said that this fact may 
ave had something to do with the peculiar germination of 
these species, and possibly it did, but Puccinta Phragmitis 
(Schum.) Korn. and Puccinia Redfieldi@ Tracy were collected 
at the same time, and after passing through the same treat- 
ment, germinated in April, also, but with the production of 
the ordinary pedicelled sporidiola; besides, Puccinza Sporobolt 
was taken from fresh out-door material. : 
I have constantly had much aid from Prof. A. 5. Hitch- 
cock, in the course of my investigations, particularly in the 
selection and preparation of the various solutions used in get 
mination. 
Kansas Experiment Station, Manhattan. 
EXPLANATION oF PLaTes XXXVII—XXXIX. 
Fig. 1. Germination of uredospores of Puccinia coronata Corda, 
carbonate (1:1000), after 48 hrs: X 247. 
Fig. 2. Condition of the sama, in potassium bi-chromate (1:1099), 
hrs. X 333. Spores much contracted and shrivel 
Fig. 3. Germination of uredospores of Puccinia 
sodium chloride (1:1000), after 21 hrs. & 247. : 
ig. 4. Germination of uredospores of Puccinia coronata, in so 
hate (1:1000), : : ination vigorous. 
P = ( ), after 52 hrs 333 eters? gc S. watch-glas® water 
in ammonium 
after 23 
dium thiosul- 
te (1;100,090)+ 
ae 
culture, after 3 days, June 15, 1892. X 333- : 
Fig. 6. Germination of the same, “in potassium bi-chroma 
after 28 hrs. X (1:1000), a 
1g. 7. Condition of uredospores of Puccinia coronatain lead acetate 
after 22 hrs. X 333. ; aban 
Fig. 8. Germination of uredospores of Puccinia Rubigo-vera, 
4 hrs. X 200. Shows the various stages in germination. 
after 
Rubigo-vera (DC.) Wint. in 
