1894. ] Myxomycetes of Central New York. 95 
of the cells remain distinct in the young plasmodium, I was 
not able to determine. But in the plasmodium there is only 
one contractile vacuole, while each of the component cells 
contained one. The movements of the mass are distinctly 
amoeboid, and the protoplasmic currents can be clearly seenin 
the plasmodium as it moves slowly from place to place. The 
expansion of the contractile vacuole is very gradual until it 
attains its full size. After remaining expanded for a moment 
it suddenly disappears entirely. The time occupied from one 
disappearance to another is from forty to sixty seconds. 
n the movement of the young plasmodium the protoplasm 
flows in a definite direction for a time, until a large pseudo- 
pod isformed. The rest of the plasmodium then flows into 
the pseudo pod. The movement is that of the whole mass in 
a definite direction. The young plasmodium meanwhile is 
irregular in outline, owing to the putting out of small pseudo- 
pods from all sides of the mass. 
I was unable to induce the development of this species be- 
yond the young plasmodial stage. Many different methods of 
culture were tried, with many different media, but all without 
Success. I was particularly desirous of obtaining a mature 
Plasmodium of. this Species, in order to study the formation of 
the ethalium. As is well known, the arrangement of spo- 
» More fortunate than myself, may be able to com- 
Ra these observations, and work out this interesting struc- 
re. 
oe acknowledgments are due to Prof. G. F. Atkinson for 
si and kindly advice in carrying out this study. 
etantcal Laboratory, Cornell University. 
pra ipPendage. — Fig. 12, biciliated swarm-cell.— Figs. 13-15, am- 
Modium,  "/8-— Fig. 16, coalescing swarm-cells.—Fig. 17, young plas- 
