1894.] Cultures of Isaria farinosa. 135 
the character of the margin of the colonies. By transmitted. 
light the elevation of the colonies could not be shown. This 
can be done by photographing in the ordinary way by re- 
flected light. Such a photograph taken on the same day is 
teproduced in figure 4, of the same plate. The margin of 
the colonies, however, is not shown by this process, but a 
knowledge of the true character of the colonies can be ob- 
tained by putting the two photographs together. 
Several cultures on artificial media in culture tubes have 
been made but in no case has any thing resulted which shows 
the perfect or ascigerous stage of the fungus. Upon nutrient 
agar, nutrient gelatine, and bean stems, nothing but the cottony 
or fluffy growth, covered by the farinaceous fructification, ap- 
Pears. On potato this growth first appears, to be succeeded 
by the characteristic fructification of the Isaria stage. 
Tulasne has shown!, not by cultural experiments, but by 
contiguity of development, that /saria farinosa (Dicks. ) Fr. 
is the conidial stage of Cordyceps militaris (Linn.) Link. A 
large number of cultures, perhaps varying the substratum 
and other conditions of environment, might result in the de- 
velopment of the Cordyceps form in artificial cultures from 
¢ Isaria stage, 
_ The fact that the Isaria stage will develop readily on var- 
pine oa as described above is evidence that it can de- 
res i uy as a saprophyte, and is thus more likely to be 
than if it In greater abundance and in wider distribution 
B “oterDiha able to propagate itself only on insects. 
al Department, Cornell University. 
EXPLANATION OF PLATES XIV-XVI. 
'8. 7, gro 8. 5, germinating spores.—Fig. 6, farther advanced stage.— 
Ural tions basidia from sporophore of plant developed under nat- 
Con 
Plate XVI a ae 8 and 9, same, from culture on agar. 
were Started: noha Photograph of Isaria farinosa which cultures 
: from 
ony on a on twice.—Fig. 11, fructification in elevated portion of 
5 plat : 
56,7,8 ad and XVI the scale shown is 1™ magnified about 18 times. Figs. 
lucida, are Magnified 50 times more than the scale. Drawn by aid of 
Note sur x 
35. les Tsaria et Sphzeria entomogenes. Ann. d. Sci. Nat. Bot. IV. 
; 1865. 
1854 : F. 
57-—Torrubia Mmilitaris. Selecta Fung. Carp. 3: 6. 
