188 The Botanical Gazette. [May, 
the Ustilago lineata Cke. is referred at present. It is a very 
common form near New Haven, upon the leaves of Zizania 
aguatica. The spores are dark and form elongated sori in 
the leaf tissue. They germinated rather freely when sown 
in water in May, 1892. The promycelium reached a length of 
from 25 to Sou. The sporidia were usually four in number. 
They do not seem to conjugate but produce buds from the 
distal end both before and after falling from the promycelia. 
RHAMPHOSPORA NyMPHA:z Cunningham is described as 
occurring in leaves of different species of Nymphea in India. 
What appears to be exactly the same thing has been found by 
the writer growing in leaves of Nuphar advena near New 
Haven, Conn., and in leaves of Vymphea odorata at Ledyard, 
Conn., and at Woods Holl, Mass. Sowings were made in 
water both from fresh and from dried material but were un- 
successful. 
Cunningham’ separates this species from the genus Enty- 
loma and makes it the type of the new genus Rhamphospor, 
because all the spores are borne at the tips of hyphal branches 
and because the promycelium is subverticillately branches 
A comparison between Cunningham's figures® and east 
the germination of the spores of Extyloma Magnusti as figure 
by Woronin%and that of the spores of Doassansia obscura as 
given by the writer!° will show that the ‘‘branches 
promycelium are indeed primary sporidia and the fac 
they do not conjugate, while the bodies produced a a 
them do, is hardly sufficient for classifying them as pecu es 
structures. Inmany of the species of Entyloma and mee 
the primary sporidia do not conjugate, yet there is no Oe 
for considering them to be promycelial branches, for ee 
arise in exactly the same way that the primary sporidia W" ae 
conjugate do. The fact that these ‘‘promycelial br anches 
finally septate is not in the way of their being cons! 
sporidia, for the primary sporidia of many species 
and Doassansia are finally septate. The regular co 
between what Cunningham calls the primary sporidia is 8 ee 
but hardly sufficientiy characteristic to be regarded as 
eric rather than a specific distinction. 
‘ $33. 
7Scientif. Mem. of the Med. Officers of the Army of India, 3- ee 
®Loc. cit. p/. 2. figs. 7-76. 
®Beitr. z. Kenntn. d Ustilagineen, p/. 4, figs. 24-26. 
1Annals of Botany 6: p/. 7. figs. 37-42. 
