* 
concerning the identification or failure to note the defim 
246 The Botanical Gazette. [June, 
In April of this year I collected a fresh specimen, and germinated 
spores from it side by side with spores from the specimens then a year 
old. I found that, as before, about one-tenth of the spores from the 
fresh specimen germinated, while one-third to one-half of the year-old 
spores germinated. The swarm-cells from the latter seemed to pos- 
sess more vitality also, remaining alive in distilled water longer than 
the swarm-cells from the fresh spores. Very few of the latter were 
found active at the end of twenty-four hours. There was little differ- 
ence in the time required for the germination of the two. The tem- 
perature was always that at which the air of the laboratory happened 
to be, no attempt ever having been made to keep the spores at any 
given temperature. 
The diameter of the spores is about 8, of the amoeboid cells about 
7M, and of the swarm-cells about 6. Only uniciliate swarm-cells 
were observed. : 
I have also germinated year-old spores of Diachaea leucopoda Bull. 
Hemiarcyria rubiformis Pers.,and Fuligo septica (Fries) Link., an 
spores of Badhamia hyalina Pers. two months old. Only a small per- 
centage of any of these germinated, but the time required was less than 
three hours for any of them. Only amoeboid cells of Hemiarcyria 
rubiformis were observed. In all cases about one-half hour was con 
sumed by the protoplasm in escaping from the epispore, and the time 
given above as the time required for germination is that between the 
moment they were placed in water and the moment the protoplasm 
assumed the swarm-cell form. 
It will be seen that my experience agrees more closely with that of 
De Bary than that of Durand. The time required for the eben! 
tion of some of the spores is shorter, however, than that of which 
find any record. I am indebted to A. P. Morgan for the determina- 
tion of the specimens mentioned in this article ALFRED jaune ae 
Ciatcuir, Biological Department, Throop Polytechnic Institute, Pasa 
dena, Calif. 
its occurrence in the United States. Wolle,* whether fro 
te terms 
vee lifor- 
Dr. Farlow’s announcement, states that “it is reported from Ca or 
d 6 
iggb® Baty, Comp. Morph. and Biol. of Fungi, etc. (Eng: trans.) 427 4% 
* Farlow, W. G., Notes on fresh-water alge. Bot. Gaz. 8: 224 _ 
* Wolle, freshwater alge of the United States. 104. 1887. 
BEAR Rc ie ae eae fe ee i) ae ea ne Oa en es 
