334 The Botanical Gazette. [August 
We prefer a rather coarse sawdust to a fine one for general pur- 
poses. The seeds should be sown in well drained pots and watered 
at least once a day. It must not be forgotten, however, that roots 
germinating in sawdust or even in moist air or water, are slightly dif 
ferent from those grown in earth. The roots of sawdust cultures pre- 
sent characteristics in their growth which are closely allied to roots 
grown in moist air.—G. E. Stoner, Amherst, Mass. 
Note on the development of a filamentous form of Protococens in en- 
tomostracan appendages. (WITH PLATE xxx.)—While examining a col- 
lection of Sph@roplea annulina brought in during the latter part of 
April, a very curious object was discovered under the microscope which 
at first sight might well have been mistaken for a new algal form. It 
proved to be fragments of the appendages of some entomostracal, 
presumably a Branchipus, in which a colony of Protococcus had ob- 
tained a foothold and was apparently in a very thriving condition. 
The plant was an aquatic form, the collection having been m 
from submerged meadow lands. It agreed in every respect with the 
description given by De Toni of Protococcus infusionum (Schrank) 
Kirchn., var. Roemerianum (Kuetz.) Hansg. The cells were of a bright 
green color, globose when free and angular in the crowded portions 
of the mass; the cell membrane was thin and the contents were homo- 
geneous; the average diameter of the cells was about lof. 
One fragment of the animal appeared to be a part of the antenna, nif 
ing two long slender sensory hairs each furnished with one row of spines 
and a single short hair bearing two rows of spines. A second bit yer : 
made out to be probably a portion of the gills. Two lobes ee 
furnished with a row of hairs about .5™ in length and 17 19 . ae 
ter. These hairs were hollow and were very similar in appeals | 
size to the two long hairs of the antenna. A mass of Prolecea . 
formed in the broad basal portions of the structures and ote - 
were so numerous that they were crowded and pressed out of vo 
tural form, becoming angular in outline and giving the ere 
a plate or layer of connected cells. Some of the cells had ‘a i 
their way up into the hairs, and undergoing division there had : mee 
entirely filled the hollow lower half of each hair. Every epee 
tained from one to four oblong masses of cells each mass being ae 
sult of the division of a single cell. ; Is lying 
The common form of Protococcus consists of single cells . each 
gether in a loose mass. Instead of this we here have filamen™ 
composed of several cells. This is apparently brought dual cells 
by the conditions in which the plant finds itself. di gave OP” 
were forced up into the hairs whose walls, being transparenb | : 
