452 The Botanical Gazette. [November, 
place them from twelve to eighteen hours in pyroligneous acid. 
Next place the objects in asolution made of one part twenty per 
cent. hematoxylin, ninety-nine parts seventy per cent. alcohol. 
Keep in the dark and leave from twelve to eighteen hours, 
and after that in the dark for some time in seventy per cent. 
alcohol. Imbed and section. After the sections are fastened 
to the slide, cover them with a solution of potassium perman- 
ganate, which has so much water that it possesses a light 
rose color, and leave until they have an ocher color. Then 
wash the sections with a solution of one part hydric oxalate, 
one part potassic sulphate, 1,000-2,000 parts water. After 
this, stain the sections for three to five minutes in a saturated 
alcoholic (100%) solution of safranin; clear and mount in Can- 
adabalsam. Lalso prepared root tipsin Flemming’s fixing fluid, 
and after imbedding and sectioning, stained first with Klein- 
enberg’s hematoxylin and then with a two per cent. aqueous 
solution of acid fuchsin. The ovaries and anthers of Lilium 
longiflorum Thunb., I stained in several ways. The centro- — 
somes and attraction-sphere will be stained quite well, how- 
ever, by simply leaving them for a considerable length of time 
in anilin-safranin, and then taking out the excess of color 
with alcohol. By another method I took equal parts of an 
aqueous (two per cent.) solution of acid fuchsin and acetic 
methyl-green, which in some cases made a very favorable stain. 
The last method tried was one suggested to me by Professor 
Newcombe: (1) a one per cent. aqueous solution of ferrous 
sulphate, (2) a five per cent. aqueous solution of tannic acid, 
(3) anilin-safranin (one part of one per cent. alcoholic solu- 
tion of safranin with two parts water), (4) an aqueous solution of 
picro-nigrosin, strong enough to have a dark bluish-green color. 
The slides holding the sections were placed thirty to forty- 
- five minutes in the iron solution, then washed in water, next, 
the same length of time in the tannin, and washed again. 
Now the sections were covered again with the iron solution 
and left for a minute or two or until they changed to a rather 
dark color. After washing off the iron in a stream of water 
they were stained in the anilin-safranin from thirty minutes 
to one hour, and afterwards fifteen, minutes or more 1 = 
picro-nigrosin. After raising them through the grades © 
alcohol and being careful so as not to take out too much of the 
Safranin stain, they were mounted in balsam. The centro- 
somes were stained very dark and the attraction-spheres © 
