482 The Botanical Gazette. ' [December, 
for twelve to twenty-four hours, or until they were perfectly 
fixed. They were then removed, washed with water, and 
carried through the different grades of alcohol up to 70%, 
where they were allowed to rest. Sections were cut and 
stained with either borax-carmine or hematoxylin, washed, 
then passed through 95% and 100% alcohol, cleared in oil of 
origanum, and mounted in balsam. For the study it was nec- 
essary to have transverse sections of the motor organ, of the 
petiole above and below the motor organ, and of the rachis; 
also median longitudinal dorsiventral sections of the organs in 
their diurnal and in their nocturnal positions. Over one hun- 
dred and fifty slides were prepared for the work. The borax- 
carmine stain was used for the majority, and it was found 
necessary to leave the specimens in the stain for about twelve 
hours, on account of the slowness with which they took it up. 
| Species studied. 
Twenty-five species representing four families and seven- 
teen genera were studied. The following is the list: 
I. LEGUMINOS#. 
Baptisia leucophaea Nutt., B. leucantha Torr. & Gray, Trt- 
folium pratense L., T. hybridum L., T. repens L., T . pre- 
cumbens \.., Melilotus alba Lam., Medicago lupulina L., 
Amorpha canescens Nutt., Petalostemon violaceus Michx., P. 
candidus Michx., Desmodium acuminatum DC., D. canescens 
DC., D. Canadense DC., Lespedeza capitata Michx., Vécta 
sativa L., Lathyrus ochroleucus Hook., L. venosus Muhl., LZ. 
palustris L., Aptos tuberosa Moench, Amphicarpea monoica 
Nutt., Gleditschia triacanthos L. 
II. GERANIACE#. 
Oxalis corniculata L., var. stricta Sav. 
Ill. POLYGALACE. 
Polygala verticillata L. 
IV. MALVACE#, 
Abuttlon Avicenne Gertn. 
Definitions. 
In the descriptions which -follow I have called th 
organ located at the base of the petiole the primary ia’ 
In the pinnate leaves the whole of the secondary petiole oO 
e motor 
