328 University of California Publications. [ ZooLocy 
clusively that, under the conditions of the experiments’ regen- 
eration from cuts through the distal region of the stem produces 
structures of the local, 2.e., distal type. 
Experiments belonging to the second category; cuts made 
through the middle region of the stem. 
I.—August 5, No. 8. Stem with 17 hydranths and terminal 
bud. 
Pedicel 1 with 5 annuli 
Pedicels 2—5 with 4 annuli 
Pedicel 6 with 6 annuli 
Pedicel 7 with 3 annuli 
Pedicel 8 with 5 annuli 
Pedicel 9 with 2 annuli 
Pedicel 10 with 4 annuli 
Pedicel 11 with 1 
Pedicel 12 with 2 
Pedicel 13 with 1 
Pedicel 14 with 3 annuli 
Pedicel 15 with 1 annulus 
9 
1 
1 
annulus 
annuli 
annulus 
Pedicel 16 with 
Pedicel 17 with 
Pedicel 18 with 
annuli 
annulus 
annulus 
10 am. Cut stem between 6 and 7. 
August 8, 3:30 p.m. No. 7 has regenerated without annuli 
immediately below the hydrotheca. 
Result: regeneration according to the distal, not the local 
type. 
I].—Auegust 5, No. 9. Stem with 17 hydranths. 
Pedicel 1 with 5 annuli 
Pedicels 2—3 with 4 annuli 
Pedicel 4 with 7 annuli 
Pedicels 5-6 with 4 annuli 
Pedicels 7-9 with 3 annuli 
Pedicels 10-11 with 2 annuli 
Pedicel 12 with 3 annuli 
Pedicel 138-17 with 1 annulus 
11 am. Cut stem between 4 and 5. 
+The stems under observation were removed from the hydrorhiza and 
rested on the bottom of flat glass dishes containing about 300 ¢.c. of sea- 
water. None of the colonies took food during the course of the experiment 
beyond what they could get from the original supply of water, which was 
not changed. There is no reason to suppose that the results depend upon 
the conditions to which the stems were subjected, for several observations 
of regeneration in nature indicated that results are essentially similar in 
the two cases. 
