182 University of California Publications in Zoology [Vol. 20 



In a number of instances tliis chromophile territory seems to be 

 definitely related to the chromatoidal mass and to proceed from one 

 end of this structure (pi. 22, fig. 23) . It appears to derive its chromo- 

 phile material, which may be diffused throughout its extent, from the 

 deeply stained substance of the chromatoidal body. Not all cysts have 

 chromatoidal bodies and not all ridges are deeply chromophile, but they 

 are generally more deeply stained than the cytoplasm. 



The point of emergence of the cytoplasmic bud is a minute circular 

 pore in the wall two or three microns in diameter. We are able to 

 find this opening only in those cysts in which the bud is present. The 

 pore is sometimes near the middle of the ridge, but is more often at 

 one end. We find no evidence of a fixed relation of the pore to a par- 

 ticular part of the ridge. The diameter of the pore is less than that 

 of the nucleus, which must therefore be elongated in passing through 

 it. The cytoplasm in the pore is sometimes more deeply chromophile, 

 as though condensed by the constriction (pi. 22, fig. 27). 



The sequence of events in the budding process is as follows : The 

 protoplasm protrudes through the pore (pi. 22, fig. 25), the nucleus 

 slips out into the protoplasmic bud (pi. 21, fig. 22), the. bud detaches 

 itself as an amoebula (pi. 22, fig. 23) , and a new bud forms and another 

 nucleus creeps out. The process is repeated until the cyst is emptied 

 of all its nuclei and as many amoebulae have escaped as there were 

 nuclei in the cyst. We have found a few instances of residual proto- 

 plasm without nuclei left in the cyst, and no evidence of plasmotomy 

 of amoebulae within the cyst. 



The evidence for this budding process is the fact that the proto- 

 plasmic bud has been found without a nucleus, that nuclei have been 

 seen at and near the pore, that more than one nucleus has never been 

 seen in the bud, that the protoplasmic bud is always relatively small 

 as compared with the original parent mass which grows smaller as the 

 nuclei therein become fewer, that a small detached amoebula with a 

 typical nucleus has been seen near the pore, and that in one instance 

 a cyst with a bud in a fresh smear after an interval of an hour was 

 found to have lost the bud. We have not as yet been able to watch 

 the detachment of active amoebulae in the warm stage. Cytolysis of 

 the bud and contents of the cyst was observed in one instance. 



The number of amoebulae produced is eight as a rule, since there 

 are normally eight nuclei in the matured cyst. There is, however, 

 evidence that the number of nuclei and amoebulae may exceed this, 

 though we have never seen cysts with sixteen nuclei. Cysts with a 



