1922 J Kofoid-Boijcrs-HiCiZii: Endamocha in Hodgl.-in's Discasr :ill 



The amoebae are often distinKuished by slight differences in the 

 stainability of the cytoplasm. In some instances, there is a suggestion 

 of deterioration, but not in all. A normal amoeba with vacuolated 

 protopla.sm, pseudopodia, and small spherical nucleus with central 

 spherical karyosome and heavy peripheral rim of chromatin is 

 shown in figures 1 and 2. A deeply stained, clouded nucleus closely 

 resembling those often found in amoebae in sections of intestinal wall 

 stained in iron haematoxylin is shown in figure 3. 



The appearance of such amoeboid cells at mitosis is shown in 

 figure 4. The nucleus of this amoeba is in the late anaphase, the 

 chromosomes have reached the ends of the elongated imcleus. where 

 their individuality is somewhat masked by overlapping and fusion. 



If we apply to this nucleus the same criterions which were applied 

 in the preceding article to dividing amoebae in the bone marrow, we 

 find that this nueleu.s meets these tests. It has, in tlie first place, an 

 intact nuclear membrane which has persisted to a late phase of mitosis. 

 In the second place, there is a meridional intradesmose on the inner 

 face of the nuclear membrane connecting the two poles of the nucleus. 

 In the third place, the number of chromosome.s is neither 24 nor 48, 

 the numbers reported for himian cells. It is apparently not over 6. 

 It is impossible to be certain of the full number because of the over- 

 lapping of these small bodies. 



We therefore conclude that the.se amoeboid cells are not human 

 cells, and that they are parasitic amoebae. In view of their similarity 

 in the active state and in mitosis to Eiidamoeha dijscnteriae and also 

 because of the coexistence of the intestinal infection by this amoeba 

 in this specific case, we infer that it is this same infection that ha.s in 

 this instance reached the inguinal gland. This conclusion opens the 

 possibility that Hodgkin's disease may he amoebiasis of the lymphatic 

 system and calls for investigation along other than morphological lines. 



