1923] Bercovitz: Endamocha coU and Coimcilmania laflcuri 371 



SUMMARY 



1. In infections bj' Endaniocba coli the cysts are present as a gen- 

 eral rule in small numbers; while in infections by Councilmania the 

 cysts are abundant. 



2. In general the cysts of Councilmania take the Congo red stain 

 whereas the cysts of E. coli either do not take the stain, or else in most 

 cases take it very faintly. 



3. Of all cysts of intestinal protozoa tested with some twenty-five 

 anilin stains, only cysts of E. coli and Councilmania took any of the 

 stains at all, and these two took only the Congo red. Furthermore, 

 only the cyst wall was stained, the dye apparently not passing through 

 the wall and thus not staining either cytoplasm or nuclei. 



4. The cysts of the common intestinal protozoa when dead are 

 quickly penetrated by anilin dyes and the protoplasm is deeply 

 stained. Living cysts resist penetration by these dyes. 



5. The shape of cysts of Councilmania in many cases is ovoidal, 

 or ellipsoidal, whereas cysts of Endamoeba coli are as a rule spherical, 

 and also slightly smaller than Councilmania cysts (see Kofoid and 

 Swezy, 1921, a, &). 



6. The condition of the stool, i.e., whether formed or unformed, 

 whether acid or alkaline, had no effect either upon the abundance of 

 the cysts or the way in which they reacted to the Congo red stain. 

 There was no evidence that in the more fluid stools the cysts were 

 less protected than in the formed stools. 



presbyteri.a.n hospital, 

 Katchek, Hainan, Chixa. 



Transmitted, December 8, 19SS. 



LITERATURE CITED 



Kofoid, C. A., and Swezy, O. 



1921a. On the free, encysted, and budding stages of Cotincilmania lafleuri, a 



parasitic amoeba of the human intestine. Univ. Calif. Puhl. Zool., 



20, 170-189, pis. 18-22, 3 figs, in text. 

 1921b. Councilmania lafleuri, a new amoeba of the human intestine. Proc. 



Soc. Exper. Biol. Med., 18, 310-312. 



