1913] Sweet: EpUhelioma of the Common Fowl 45 



Table III 



Table of Complement Fixation Amounts in Cubic 

 Data Centimeters 



I II 



Suspected Sheep cells, 



Na CI seriun Antigen Alexin^ Amboceptor suspensio 



1 1 0.1 1 1 ce. 1 cc. 1 cc. 



2 2 0.1 .. 1 cc. 1 ec. 1 cc. 



3 1 0.2 1 1 cc. 1 ec. 1 ec. 



4 2 0.2 ..' 1 ee. 1 ec. 1 ec. 



5 1 ... 1 1 cc. 1 ce. 1 cc. 



6 .. .... 2 1 cc. 1 ce. 1 ce. 



7 2 ... .. 1 cc. 1 ec. 1 ce. 



8 2 .... 1 1 ec. 1 cc. 



9 .3 ... .. 1 ce. 1 cc. 



10 4 .... .. 1 cc. 



1 Incubate one hour after thorough shaking. 



= Incubate 5 to 10 hours after thoroufrh shaking. 



Variations in Amounts of Suspected Serum Used. — By adding 

 additional tubes corresponding to tubes 1 and 2, 3 and 4, the 

 quantities of the suspected serum were varied, being added in all 

 quantities from 0.1 to 0.8 cc. The antigen was found no longer 

 to prevent haemolysis at a dilution of 1 to 100, so was used at 

 a dilution of 1 to 200. The alexin usually produced complete 

 haemolysis at a dilution of 1 to 20 or 1 to 25. The amboceptor 

 was used at a dilution of 1 to 1000, and the sheep cells in a 5% 

 .suspension in physiological salt solution. 



Complement Fixation. — With normal serum in quantities 

 from 0.1 ccm. up to 0.8 ccm. haemolysis was complete. Haemolysis 

 was usually complete after two hours, and always at the end of 

 five hours ' incubation. The same result was obtained with serum 

 taken from infected fowls during the period of incubation. How- 

 ever, a different result was obtained with serum from fowls in 

 which the lesions of epithelioma contagiosum were present. In 

 this serum there was considerable evidence of a specific antibody. 

 Haemolysis did not occur as readily and was almost constantly 

 incomplete at the end of ten hours incubation. The results were 

 not as striking as we believe they could be made by variation 

 in the nature of the antigen, and since there is no question of 

 diagnosis involved will never be of practical value, but it is cer- 



