Euglena Viridis (Ehrenberg.) 
By SIstER M. ELLEN. 
This flagellate was found in unusual abundance early in January, 
1921, in a little spring-fed creek which remained open all winter. 
This creek is the watering trough for cattle. and other farm animals 
and the water is more or less polluted as seems to be the characteristic 
condition under which Euglena viridis grows in abundance. About 
the middle of January, it was observed that the bottom of this creek 
was completely lined with the rather gelatinous, green masses of 
Euglena cells. This condition continued all winter and, at the sug- 
gestion of Dr. Bert Cunningham, the following investigations were 
made: 
Test of Evolving Gas 
On April 12, much inflated masses of the Euglena cells were floating 
on the surface of the water. A quantity of the material was col- 
lected and a test of the evolving gas proved it to be oxygen. Masses 
of the material, especially after they had stood in the laboratory for 
a few hours, invariably emitted a decidedly ‘fishy’ odor. Such an 
odor is mentioned by Butschli in describing E. sanguznea, 
Tests for Chlorophyll 
After a quantity of the material had stood for several hours in a 
large stender dish exposed to the light, the thick, green, oily layer of 
Euglena cells was-poured off and allowed to filter, the cells remaining 
on the filter paper. The filter paper was then transferred to a 
beaker of methyl alcohol which was heated to the boiling point over 
a water bath. 
In order to check the results of the test another alcoholic extrac- 
tion was prepared in the same way from fresh parsley leaves. After 
the extraction from the Euglena cells was complete, the green liquid 
was put through a filter,-leaving the cells on the filter paper. These, 
when examined under the microscope, showed practically none of 
