150 THE AMERICAN MIDLAND NATURALIST 
their original color, but were intact and but little shrunken by this 
treatment. The separation of the pigments of the chlorophyll extrac- 
tions, both of the Euglena cells and of the parsley leaves was done 
by adding to about 20 c. ec. of each of the extracts the Same amount of 
benzine. The vials were then shaken vigorously and their contents 
allowed to stand until the pigments separated. The yellow alcoholic 
layer and the blue-green benzine layer were identical in the two 
extracts. The flourescence, too, both of the chlorophyll solutions and 
of the cyanophyll-benzine layers were the same in the Euglena and 
the parsley extractions. Tests for the effect of sunlight and of 
darkness on both extracts gave corresponding results. 
Some of the Euglena cells were boiled in water for a minute or 
so. No pigment of any kind discolored the water and the cells 
themselves, when examined under the microscope, shawed no color 
change and but little distortion or shrinkage. It was observed, how- 
ever, in the cells subjected to this treatment, that the flagella were 
made quite prominent. 
Cultures 
Such media as tap water,* creek water, (filtered and unfiltered) 
Marschal’s solution, Byrenck’s solution, very dilute solutions of cane 
sugar, prune juice, egg white, egg yolk, whole egg, and milk, all 
tried in darkness and in light under the same conditions of tem- 
perature, gave results, that is, motility, growth, and reproduction, 
incomparably better in the light than in the darkness. The best 
results obtained from the inorganic nutrients, creek water, and tap 
water, in moderate light. These results were all about equally good. 
The second best results were in the organic nutrients in the light. 
The cultures which ranked as a distant third were those in the 
organic nutrients in the dark. In these, a comparatively few cells 
divided longitudinally after a few days, but there was little motility. 
The chloroplasts in these cells, in general, did not disintegrate any 
more rapidly than did the cells themselves. A very few motile cells 
showed a number of non-green plastids. 
Among the hindrances in the cultural work, are the numerous pro- 
tozoan forms which thrive in the cultures about equally well in light 
and darkness such as species of Paramoecium, Vorticella, Stylonychia, 
* This is deep well water which, on chemical analysis, yields calcium, 
iron, magnesium, the hydroxyl, sulphate, and carbonate radicals, and a - 
_ trace of chlorine. 
