I 



lfil5] Sirezy: On a New Tricliomonad Flagrllaie 91 



the daughter blepharoplasts move apart both undulating membranes 

 are found well developed and nearly equal in size (pi. 12, fig. 5). 

 The behavior of the chromatic basal rod or parabasal body has not 

 been quite clear, but since the second one, when iir.st observed in the 

 dividing cell, does not take a dark stain with iron haematoxylin. this 

 reaction appearing later, it is probably a new outgrowth, as is the case 

 with the same organelle in the triehomonads. 



The new flagella begin to make their appearance as new outgrowths 

 as soon as the blepharoplast has divided (pi. 12, fig. 5). two of the old 

 flagella going to one daughter blepharoplast and one to the other. 



With the beginning of mitosis a rearrangement of the chromatin 

 takes place (pi. 12. figs. 5, 6) . The karyosome breaks up into a number 

 of granules (fig. 4) and these later become arranged into a short skein 

 or spireme (fig. 5). The breaking up of this into a definite number 

 of chromosomes was not very clear, but indicati(ms of this are found 

 in figures 6 and 7. As shown in tlie late telophase stage of division 

 (figs. 9, 12). the nmnber of chrcjmosomes is apparently two. Some 

 indication of a larger number is found in figure 7, but it is quite 

 possible that this indicates rather a precocious splitting of the chromo- 

 somes as in Trichomonas (see Kofoid and Swezy, 191oa). 



Division of the nucleus takes place within the nuclear membrane. 

 The formation of a definite spindle has not been observed, but a 

 comparison of the several stages shown with the corresponding stages 

 found in Triclunnonas and allied forms (Kofoid and Swezy. 1915&) 

 will at once show the very close resemblance of the process in Tricho- 

 mltits to that described for other triehomonads and is suggestive that 

 the missing stages will be found to resemble the corresponding .stages 

 of the other species. This point, however, is not insisted upon. It 

 is sufficient to call attention to the striking similarities in the division 

 cycles of all the members of the three genera referred to, namely, 

 Trichomonas augusia, T. muris, Tetratrichomonas prowazeki, and 

 Eutrichomastix lacertae, and leave it to future investigation to supply 

 the missing details. 



Throughout mitosis and even after the completion of the division 

 of the nucleiLS and the separation of the two daughter nuclei the 

 blepharoplasts remain connected by a darkly staining line, the para- 

 desmose (pi. 12. fig. 9). It lies outside the nuclear membrane and is 

 apparently attached only to the blepharoplasts. This line gradually 

 loses its staining reactions and disappears. 



