VII. EXPLANATION OF PLATE 14 

 Trypanosoma triatomae sp. nov. from stomach of Neotoma fuscipes. 

 All figures were outlined with a camera lueida using a 1/16 Leitz objective 

 and Zeiss ocular 12. The magnification is in all cases approximately 3195. 



Figs. 1-8. Large, broad trypanosomes from stomach of a bug about four 

 days after possible infection. Giemsa stain, wet method. 



Fig. 1. Parabasal in extreme posterior end showing a broad, bar-shaped 

 structure stained deeply at ends. Blepharoplast small. Nucleus elongate, in 

 characteristic position at concave side close against the edge, numerous heavily 

 stained granules within nuclear membrane. Prominent undulating membrane 

 the entire length of the flagellum. 



Fig. 2. Large, broad trypanosome having large, uniformly stained parabasal 

 a short distance from posterior end. The fan-shaped suspensory structure, or 

 parabasal rhizoplast, quite clearly shown, nucleus elongate, granular, asymmet- 

 rical. Small blepharoplast. Faint chromatic line (axostylef) extends from 

 blepharoplast to posterior end. 



Fig. 3. More slender trypanosome, with small parabasal and extremely long 

 nucleus. 



Fig. 4. The same as figure 2, with myoneme present. 



Fig. 5. Shorter, broad form showing evidence of the shifting of the ble- 

 pharoplast and parabasal anteriorly. The parabasal shows unequal staining in 

 periphery and center. Small blepharoplast and clearly defined parabasal 

 rhizoplast. 



Fig. 6. The same, with a chromatic line extending from blepharoplast to 

 posterior end, terminating in a very small granule. 



Fig. 7. Large, slender trypanosome with a large, square, parabasal with 

 fan-shaped rhizoplast and slightly elongate nucleus. 



Fig. 8. An exceedingly large trypanosome. The blepharoplast and para- 

 basal have migrated about one-half of the distance between posterior end and 

 nucleus. 



Figs. 9—12. Merozoite-like forms from the stomach. Bodies more elongate 

 and narrower. Nuclei narrower, showing great numbers of small granules. 

 Parabasals small, compact, deeply stained; blepharoplasts and parabasal rhizo- 

 plasts not distinguishable. Schaudinn-iron haematoxylin, wet method. 



Fig. 13. Crithidial form from stomach, showing transformation from try- 

 panosome to crithidial form. Note trypanosome-like shape of body, but ble- 

 pharoplast and parabasal body have migrated to a position opposite that of 

 the nucleus. Nucleus broadly ellipsoidal but still diffusely stained; a distinct 

 parabasal rhizoplast. Faint axostylar thread along edge of body. Giemsa stain, 

 wet method. 



Figs. 14, 15, 16, 18. Large crithidial stages from stomach. Conical or blunt 

 posterior end. Large, vesicular nucleus, with distinct nuclear membrane, cen- 

 tral karyosome and radiating fibers. Parabasals relatively small, having a 

 slight bilobed appearance on upper edge. Parabasal rhizoplasts distinct. Small 

 blepharoplasts. Figures 14, 15, 16 show axostyles extending from blepharoplast 

 to posterior end. Figure 16 also shows distinct rhizoplast connecting blepharo- 

 plast with karyosome of nucleus. Note lateral attachment of parabasal to 

 blepharoplast in figures 14 and 15. Schaudinn-iron haematoxylin, wet method. 



Figs. 17, 19. Crithidial forms in stomach leading toward rolled-up stage. 

 Yig. 17 — Body bent, but cytoplasm has not yet massed about nucleus. Fig. 19 — 

 Massing of cytoplasm about nucleus as result of the progressive bending of 

 body. 



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