166 Humbert. 
In the early spring of 1909 seeds were started in the green house 
in sterilized soil as follows: (1) all the seeds from injected capsules, 
planting the seeds from each capsule in a separate flat; (2) seeds 
from six capsules each from plants 250—II, 250—12, and 250—13, 
planting the seeds from each capsule in a separate flat. These six 
capsules were from different parts of the mother plant and all under 
bag. (3) seed from bagged capsules from plants 250—1, 250—2, 
250—5, 250—6, 250—7, 250—8, 250—-I0, 250—II, 250—I2, 250—13, 
250—I4, 250—16, and 250—20. In this latter case the seeds came 
from separate capsules on each plant and these were mixed together. 
The seeds from each plant were planted in a separate flat. As soon 
as weather conditions would permit the young plants from the in- 
jected capsules and the mixed seed from individual mother plants 
250—I, 250—2, etc., were taken to the plant breeding garden and 
set out in rows on a very uniform plot of ground. The young plants 
were one foot apart in the row and the rows were three feet apart. 
Very few died and the plot received thorough cultivation so that the 
plants of the 1909 crop had uniform environmental conditions to a 
remarkable degree. The progeny from separate capsules of mother 
plants 250—II, 250—12 and 250—13 were similarly transplanted 
on another plot of ground known as the „Mitchell Farm“ and 
equally well taken care of. It does not seem necessary to give 
a map of the plantings. It might be well, however, to restate the 
system of pedigree numbers used with special reference to the 1909 
(third generation) crop, since from this crop most of the data are 
taken. The lines from injected capsules were all planted in the plant 
breeding garden. Each line is numbered to represent the grandmother 
plant, the mother plant, and the injection number. For example, 
250—11c671 shows the line to have come from grandmother plant 
250, from mother plant 250—11, and from a single capsule injected 
with chemical number .671. By turning to the table of injections 
we see that the chemical used (671) was NaCl, that it was of 1/,0% 
strength, and that the bud was very small when treated. Individual 
plants of this line would have one additional figure added to the 
pedigree number thus: 250—ıIIc67I—I or 250—11Ic671I—13 etc., 
according to the position of the plant in the row. 
The lines from capsules not injected from mother plants 250—1, 
250—2, etc., were all planted in the plant breeding garden and are 
numbered 250—Ia, 250—2a, etc. Individual plants receive one addi- 
tional figure thus: 250—Ia—I etc. 
