Studies on the Variability and Heritability of Pigmentation in Oenothera. 351 
Table 8. 
Race No. 25. Io plants observed in 1909. 
Pedigree Number | | | | - 
of Plants | 2 | S | 3 Aa 6 2 5 
I 
1.9 za. — | = — | 77.5 34.5 10.5 51 
1.12 - | -| - | -| —- | 3 13 13.5 | 1.51) 
2.2 — = | = 5 = 58 33 Ir | — 
4.4 = - —- — 1.0 23 21 ry SS 
5.6 = || 7:0 5.5 26 14-5 —_ — 
9.7 ee ey ee = ar L I Bi |e 
10.3 = — | = 16.5 || pee) 21 7 
5-3 - | - | - | - | - | ı | -| ma | - 
9.1 _ | — — — | 3.5 | 20.5 19 11 _ 
5.42) EU IS lhe (i ale =| — 
— = 50 30:55 ko. | 220.0 | 157.0 | 196.0 | 2.0 
— Su soos 395 196 | 2 
This race (No. 25) therefore shows a decided tendency to produce 
a slightly greater amount of pigment than O. rubrinervis, although 
the modal condition is the same as in O. rubrinervis. Certain indi- 
viduals which show somewhat less pigmentation, also approach O. rubri- 
nervis more Closely in several other respects. 
In 1910 there were grown 154 plants of the next generation of 
race No. 25, and although my absence prevented statistical records 
being kept, it is certain from my own observation that the plants 
came true in pigmentation as in other characters, showing as before 
a slightly greater amount of pigment than is present in O. rubrinervis. 
Similar observations were made on the sepals of several other 
forms. In O. laevifolia, in plants grown at Wood’s Hole, Mass. in 
1908, a wide range of color variation was observed, the position of 
the mode varying in different individuals. Thus in plant No. 2 the 
mode was found to be 7 (4—5), for No. 6, 2(4), for No. 9, 3(4), No. II, 
(I—2)4, No. 15, 4—6. The same range of variation was therefore 
observed as in O. rubrinervis. The color patterns of the offspring of 
some of these plants are given in tables 9 and Io, for comparison 
with the parents. 
1) On four buds the hypanthium showed a pale tinge of red. 
2) This plant and several others of this culture departed from the type of the 
race in having, in addition to a slightly developed red color pattern, larger buds 
bearing more numerous long hairs on ovary, hypanthium and cone, and lighter green 
leaves with white midveins. In all these characters these plants approach more 
closely to O. rubrineruss. 
