308 BOTANICAL GAZETTE | OCTOBER 
perithecia were then past maturity and many had discharged 
their spores. Closely associated with the perithecia were numer- 
ous pycnidia, appearing as much smaller rounded black bodies. 
A dilution culture was made in potato agar from the ascospores 
which were supposed to be free from the pycnospores. The 
ascospores soon germinated and camera lucida drawings of the: 
germinating spores were made (fig. 7). Numerous colonies now 
appeared in the plates. They were circular, showing radiations 
from the center, and in concentric rings, a little later, the begin- 
nings of perithecium like bodies appeared. These colonies were 
then transferred to infusions of bean stems in tubes where they 
continued to develop. Contrary to expectation, however, when 
the round black perithecia like bodies matured they contained 
not asci, but pycnospores. The pycnidia were rounded or oval 
in form, opening usually by two apertures (sometimes by more, 
sometimes by only one), and extruding their spores in the typical 
worm like manner. The spores are fusoid-elliptical, hyaline, 
continuous, about half the diameter of the mycelium. In the 
colonies the pycnidia appear first near the center and may be 
found in all stages of development along a radius of the colony, 
the youngest stages nearest the circumference. The beginnings 
of these structures are shown in figs. g and 5. No pycnospores 
had been observed in the agar plates in which the sowing ih 
made, and since the ascospores were known to have germinated, 
the natural conclusion was that ascospores had produced 
pycnidia, but this conclusion was found to be doubtworthy 
later. Meantime, there had appeared in the bean tubes, et 
ciated with these pycnidia, a grayish white m ycelium with erec 
branching hyphe upon which were borne multiseptate a 
brown. Although these were normally 
hyphe, as true conidia, they sometimes appeared as 1 
growths in the mycelium. When these conidia wert ies 
mature a sowing of them was made in potato agat- - 
ce er 
