310 BOTANICAL GAZETTE [OCTOBER 
was hoped by this means to get all the successive stages of 
development (see account of sections below). Transfers of these 
larger colonies were also made to the bean stems in tubes, where 
they continued to develop perithecia and March 5 a culture was 
made from the ascospores contained in these perithecia. These 
spores, although full grown, were still hyaline in color, but they 
possessed unusual vigor. The colonies grew very rapidly and 
developed to maturity. Perithecia only were produced and the 
Same was true in a third generation of this series. Another cul- 
ture was now made from the pycnidia and conidia but again they 
produced only their own kind. This failure to connect the asco- 
spores with the other forms led to the conclusion that they are 
probably distinct forms. The ascospores obtained from the oak 
in the fall germinated but failed to produce thrifty colonies, prob- 
ably because they had lost vitality or because they were not 
readily adaptable to artificial conditions. Those sown in the first 
cultures very probably germinated and then died, as did those 
transferred to the ten tubes. The pycnidia found in these first 
cultures no doubt originated from pycnospores which were ae 
ciated with the asci but overlooked in making the culture. This 
might easily have been the case, since the pycnospores are very 
small and scarcely distinguishable in potato agar. The study of 
the pycnidia and conidia has been included here because of 
interesting points of resemblance in the development of the pyc 
nidium and the perithecium, and also because in nature the tw? 
forms are found so intimately associated. Indeed their connec- 
tion is not disproved, although it has been impossible to estab- 
lish artificially a life cycle including the three forms. 
The ascigerous colonies which were finally obtained started, 
as has been said, from fragments of the mycelium of peritheci®, 
and the ascospores thus obtained, being fresh and vigorous ee 
perhaps also somewhat inured to conditions, germinated readily 
and reproduced themselves. This is further evidenced by ihe 
fact that during the winter two or three attempts were made - 
get cultures of the following species from herbarium spe 
T’. trimorpha, T. Susispora, T. aspera, and T. nitida. The first anal 
