SS a te aa eh ee le en es ae ee ie aS 
1896] CERTAIN PYRENOMYCETOUS FUNGI 315 
stained bodies at the foci of the ellipse are similar in structure. 
This indeed seemed at first to be true, since no differentiation 
resulted from the use of the ordinary differential stains. On the 
other hand, it seemed quite as possible and more probable that 
the original dark spot of the nucleus isthe nucleolus. Ina rest- 
ing condition this alone is visible, but preparatory to division 
the nucleolus moves off to an eccentric position, the hyaline 
court elongates, and the chromosomes accumulate to form the 
second chromatin mass in the opposite end of the ellipse. 
With more exact staining methods and greater magnification this 
possibility proved to be the fact. The resting nucleus contains 
a large nucleolus in the center of the cell sap. In this sap in 
some cases can be seen an indistinct linin network of very fine 
threads (fig. 27). Preparatory to division the nucleolus becomes 
€ccentric and the circular space elongates. The linin now 
becomes more distinct, the threads having grown thicker at cer- 
tain points (fig. 28). In the next stage small deeply stained 
chromatin bodies (not more than four have been counted ) appear 
at the end of the ellipse opposite the nucleolus, arranged in what 
might be the equatorial plane of a spindle, but no spindle threads 
have been distinguishable in any case. At the poles of this 
plane were two small rounded bodies which were probably cen- 
‘tospheres, although no radiating stria could be seen (fig: 29). 
Again, two groups of chromosomes, of four each, were found near 
the positions previously occupied by the centrospheres ( fig. 30). 
By this time the nuclear membrane had become partially dis- 
Solved. The nucleolus was now vacuolated and less deeply 
stained and lay partially out in the surrounding cytoplasm. The 
c-ll sap was still present but constituted a somewhat distorted 
mass, and in it were the outlines of two new hyaloplasmic circles 
ies — a group of daughter pe css ge 
thi ead ; : ascus contained two new nuclei, somewhat sm ai 
ee oe but having the same structure (fig. 37): nee 
a ue ‘vision was observed. Upon these cases, ” - 
cee en illustrated from camera lucida crewing may fi 
the conclusion that the nuclei in these asci divide karyo 
