318 BOTANICAL GAZETTE [ocToBER 
fruit so rapidly and in such abundance that it seemed a specially 
favorable subject for developmental study. The large dark brown 
spores in germinating extruded an endospore through a tiny pore 
at the more pointed end of the spore. The endospore contained 
a large vacuole and many nuclei, and from it arose one or more 
germ tubes (fig. 32). These germinating spores have been drawn 
large to show the nuclei, which are very distinct, occurring usually 
in pairs, but so small as to require great magnification in order to 
be seen at all. Spores sown in potato agar or upon bean stems pro- 
duced colonies of whitish mycelium, sending out from the center 
strong primary radiating filaments which become plumose at 
their tips. 
The colonies spread flat upon the substratum, and when 
grown in tubes the radiating threads pushed far out upon the 
glass, bearing fruit at some distance from the medium upon 
which the spore had germinated. This fact proved of great 
advantage in the later microscopic study, since the thin welt 
of mycelium grown on slides in Petri dishes could be easily 
transferred to the stage of the microscope and afforded excellent 
opportunity for study of the material in the best possible condi- 
tion. The mycelium was comparatively scanty and no conidia 
or pycnidia appeared at any time during the many successive 
cultures that were made. The necks of the perithecia furnished 
a noticeable example of heliotropism. Those grown in tubes 
standing in boxes, and hence lighted only from above, turned 
strongly upward. A tube was then wrapped in dark gob 
leaving only about an inch at the bottom exposed to the light, 
and suspended by a string. In this the necks turned sharply 
downward. Those grown in plates inclined always towards the 
window. 
The first microscopic study was directed specially t 
determining, if possible, whether there exists here any 5% 
organs such as have been described by Woronin (9g) for the closely a 
related genus Sordaria. For this purpose the fungus was int - 
upon bean stems and prepared for sectioning in collodion. Var S 
ous methods of fixing and staining were tried, but nothing com” 
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