1896 | CERTAIN PYRENOMYCETOUS FUNGI 323 
open. Here, as in Teichospora, no binucleated cells were dis- 
covered, but it was also true, as in the former case, that either 
because of the density of the protoplasm in these cells, or 
because of some fault in manipulation, no nuclei at all were dis- 
covered in any of these cells. As has been said, the only dif- 
ferentiation is a slightly deeper stain in a certain group of cells, 
the position of which corresponds with that from which the asci 
arise. It may be argued that only this group of deeply stain- 
ing cells are formed by division of the archicarp, but if this were 
true we should certainly expect in some cases to be able to trace 
the outlines of the fertile hypha. Moreover this latter theory 
would fail to account for the existence of the sterile cells. The 
marked difference in structure between these cells and those of 
the wall make it highly improbable that both arise from the 
enveloping hyphe. Then, too, when the sections become muti- 
lated by the rolling of the collodion or by rough handling it is 
a noticeable fact that this central tissue breaks out intact, leav- 
ing the inner surface of the wall quite smooth. This theory of 
development tends also to harmonize what first appeared to be a 
very peculiar condition in Teichospora. _The archicarp in Teicho- 
‘pera is not fertilized and is not specialized in form, but the 
further Processes of development are essentially homologous. 
here remains only to prove that the mother cells of the asci 
contain two nuclei which fuse before entering the ascus in order 
to establish a line of development analogous not only to that 
described for Sphzrotheca but also to what is found in many 
rases in the Floridew. This intervention of vegetative tissue 
between fertilization and the production of spores, provides a 
‘porophyte phase in the life cycle of these fungi which has not 
hitherto been known. 
Sinladg the deeply stained group of cells in the central par- 
yma the asci arise as papillate outgrowths, densely filled 
roan e rot oplasm and staining much more deeply than the eal 
ity tissue. Their bases lie close together and the 26 
hag long Narrow-cylindrical and very numerous cu : 
ward the ostiolum. Their protoplasm is densely gran 
