330 BOTANICAL GAZETTE —— Tocrower 
conidiophores were formed only where hyphz escaped at the sides and 
grew freely upward. When the slips were placed at greater heights 
(3-7™"), a greater development of conidiophores followed with a cor- 
responding decrease of organs of attachment. 
Next slips of mica were perforated with the point of a fine needle 
and placed at various heights as in the above cultures, with the result 
that organs of attachment were formed where hyphez came in contact 
with the overhanging surface, and conidiophores where they grew 
opposite or through the perforations. The fungus seemed to be 
entirely indifferent to light and gravity, and the formation of organs 
of attachment to be conditioned solely by the contact of hyphe with a 
hard surface. é 
The development of the conidiophores and organs of attachment 
throws still farther light on their relation to each other. For the study 
of the developing conidiophores rather old perianths of Lilium 
candidum were inoculated with conidia from a clean culture and placed 
in moist chambers. In the course of three or four days the entire 
substratum was covered by the fleecy mycelium. From time to time 
this was examined until all the stages of development were obtained. 
Briefly stated they are as follows: Strong erect hyphe, rich in prot 
plasm, branch two or three times, and from these branches repeated 
secondary divisions are sent out (fig. 2). Ultimately the apices of the 
branches swell, and peg like protuberances ( fig. 2 @) appear wh mane 
idly increase in size and number so that in a comparatively short wa 
mature ovoid conidia (jig. 2 4) are developed from them. Bs . 
formation of conidia was in progress at 3 o'clock P.M. April i 
Thus, with respect to the time of spore formation, this fungus differs 
from the one described by Klein." 
For the study of the successive stages of development * i" 
organs of attachment, the following agar-agar cultures were prepy : 
malt solution, 2 per cent.; malt solution, 3 per cent; Solow: i 
decoction, 2 per cent. ; potato decoction, 2 per cent.; gTape ee 
per cent. In all of these media the fungus grew luxuriantly, as 
ing organs of attachment when contact was possible, and con! he 
phores when this was impossible. The actual development of t 
organs of attachment was traced from a single conidium sown 1n ? r 
cent. potato agar medium, as follows: 
“Ueber die Ursachen der ausschliesslich-nachtlichen Sporenbildun 
cinerea. Bor. Zeit, 43:6. 1885. 
g von Botrytis : 
