78 TRANSACTIONS OF THE 
TABLE I. 
Effect of Renewed vs. Unrenewed Distilled Water 
on the Growth of Peas and Horse Beans. 
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Esk ELE hee PR Le = SCA ES OS ERS SE 
Medium Green Wt. of tops Dry Wt. of roots 
in grams. in grams 
PEAS: 
Distilled water 
renewed 28.55 1.131 
Distilled water 
unrenewed 28.08 1.259 
HORSE BEAN: 
Distilled water 
Distilled water 
99.56 2.772 
renewed | 110.30 3.315 
unrenewed | 
These results therefore indicate that the so-called 
injury to plants in distilled water cannot be entirely or 
even satisfactorily explained on the basis of: extraction 
of solutes from the plant tissues. If that were the case 
we should have the greatest injury and least recovery in 
those cultures in which the distilled water was renewed, 
the periodically renewed water effecting in toto a greater 
exosmosis of the salts than the water that is not 
renewed, as will be shown later. 
Growing these plants in distilled water for varying 
periods from one to twenty days, in half of the cultures 
the water being renewed every four days and not 
renewed in the others, and then transferring them to full 
nutrient solution, showed that in general better recovery 
resulted in the full nutrient solution in the case of those 
plants whose distilled water medium had been renewed. 
The period of five to ten days was found to be the limit 
of time peas and horse beans could remain in distilled 
water and then recover normally and continue their 
growth in the usual way. They could of course be 
kept in distilled water for much longer periods than that 
