246 TRANSACTIONS OF THE 
CONCLUSION: 
1. Addition of manure resulted in an increased 
number of organisms. 
2. The three groups were not affected to the same 
extent. 
8. Actinomyces group was affected to the greatest, 
and the Spore-Formers to the least extent. 
4. Younger the manure added, greater were the 
numbers of Actinomyces and Non-Spore-Formers. The 
percentage of increase was the greatest in the case of the 
Actinomyces. 
5. These results, together with the works of pre- 
vious investigators suggest the idea that Actinomyces 
are probably associated with decomposition of organic 
matter. 
