A Nezv Species of Treiuatode 3 



In all ninety-three trematodes were obtained of which five were 

 from the urinary bladder, four were from the lungs and eighty- 

 four were from the intestine; one hundred and seventeen nema- 

 todes, all from the stomach and duodenum, were obtained. Of 

 the eighty-four trematodes from the alimentary canal, eighty-two 

 were of one type and the other two were of two different species, 

 though one of these closely resembles the first type. In all, five 

 species of trematodes were found in the turtles, and will be 

 designated as types A, B, C, D and E. The nematodes were ap- 

 parently of one type. It is of interest to note the entire absence 

 of cestodes in these turtles. 



The table on page 196 shows the class of parasite found, the 

 organ infected and the degree of infection for each turtle. 



It will be seen from the table that, with one exception, all 

 of the turtles harbored parasites which were evenly divided 

 between the trematodes and the nematodes. Of the trematodes, 

 tvpe A predominated, being found in twelve out of the six- 

 teen turtles examined. The largest number of parasites found 

 in any one turtle was twenty-three, three turtles having this, 

 number. Turtle number \T harbored the greatest number of 

 trematodes, namely twenty-two, all of the same type. 



The nematodes were killed by dousing them quickly with cold 

 lacto-phenol solution in which they were allowed to remain in- 

 definitely, or killed in Zenker's fluid and preserved in eighty-five 

 per cent. (85 per cent.) alcohol. The trematodes were killed in 

 three ways, as follows : by flattening between glass slides in 

 Zenker's fluid, by dousing with Zenker's fluid and by dousing 

 with lacto-phenol solution. About two thirds of them were 

 killed by the first method, i. c., flattening in Zenker's, to be used 

 in making toto mounts. All the rest except one, were killed by 

 dousing with Zenker's, to be used for making sections. One was 

 killed in lacto-phenol solution with the purpose in mind of pre- 

 serving the eggs in as nearly a normal condition as possible. 

 Those killed in Zenker's were permitted to remain in this fluid 

 for from twelve to twenty- four hours and were then treated with 

 seventy per cent. (70 per cent.) iodized alcohol. 



Twenty specimens were mounted in toto. These were stained 



195 



