A New Species of Tr em at ode 5 



Summary of Table 

 Number of turtles examined i6 



Parasites Number Organ 



Trematode A 82 Intestine 



Trematode B i Intestine 



Trematode C i Intestine 



Trematode D 4 Lung 



Trematode E ...^ 5 Bladder 



Total No. Trematodes 93 Intestine, lungs, bladder 



Nematodes 92 Intestine and stomach 



in three stains ; some in borax-carmine, some in carmalum, and 

 others in Delafield's haematoxylin. In staining, the regressive 

 method of over-staining was always used, the material being left 

 in the stain from twenty-four to forty-eight hours, then de- 

 stained until well differentiated in seventy per cent. (70 per cent.) 

 acid alcohol. In dehydrating, all specimens were permitted to 

 remain in the higher alcohols (95 per cent, and 100 per cent.) for 

 several hours. From one hundred per cent. (100 per cent.) 

 alcohol they were placed in a mixture of one part absolute alcohol 

 and one part cedar oil, in which they remained from two to four 

 hours. They were then placed in a light clearing reagent consist- 

 ing of a mixture of cedar oil and oil of bergamot for an equal 

 length of time, then into a heavy cedar oil from twelve to twenty- 

 four hours and mounted in thin balsam. 



This procedure of changing gradually from a thin clearing to a 

 heavy clearing reagent then to a light balsam was necessitated by 

 a peculiar tendency on the part of these worms. At first an 

 attempt was made to clear them in xylol but it was found that in 

 a few hours they became perfectly opaque in the balsam. Then 

 light oils such as bergamot and a mixture of cedar oil and oil of 

 bergamot were tried but with the same result. I then tried plac- 

 ing them in half and half balsam and xylol, and permitting the 

 xylol to evaporate, thus thickening the balsam, but this also 

 proved a failure. Then, on trying the procedure just outlined 

 the clearing was successful in a majority of cases, though a few 

 have since become opaque. In experimenting thus, I observed a 

 specimen while it was becoming opaque, and found that after 



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