ON ANASSTHETICS. 307 
1) 
in MINN fl 
Te i c tk . 
i 
re 
HAH 
itt 
Hi) 
Effect of a 2°5 per 1000 solution (by volume) in normal saline of the following 
mixture :— 
Chloroform : : é : . 2 volumes 
Ether i 
” 
N.B.— In each of these three records, reading from right to left, there are three 
groups of contractions, viz. :— 
1, A group of normal contractions with the muscle immersed in saline. 
2. A group of declining contractions with the muscle immersed in the test- 
solution. 
3. A group of rising contractions with the test-solution replaced by normal saline. 
APPENDIX IV. 
The Comparative Power of Alcohol, Ether, and Chloroform as measured 
by their Action upon Muscular Contraction. By Dr. A. D. 
Water, F..S. (Royal Society, June 24, 1909). 
The object of the experiments described in this paper was to determine 
the relative physiological power upon muscular contraction of chloro- 
form, ether, and alcohol at various dilutions. 
The conclusion derived is to the following effect :— 
By volume . - lee. chloroform=15 e.c. ether=75 c.c. alcchol 
By weight . . lgrm.chloroform= 8 grms.ether= 40 grms. alcohol 
By molecules. . 1 mol. chloroform=13 mols. ether =100 mol. alcohol. 
APPENDIX V. 
Quantitative Estimations of Chloroform in Blood. 
By J. A. Garpner and Dr. BucKMASTER. 
The Anaesthetic and Lethal Quantities of Chloroform in the 
Blood.—The method we have pursued in these experiments was as 
follows: In all cases the percentage of chloroform vapour in the in- 
spired air was determined for each separate experiment. The amount 
of chloroform in the blood was estimated by the difference in chlorine- 
content of the blood before the administration of chloroform, and again 
at any subsequent period from the time when the chloroform-and-air 
mixture was inhaled. Numerous control experiments were made in 
order to ascertain whether the percentage of chlorine normally present 
in the blood of an animal remains sufficiently constant during a pro- 
longed experiment. This was found to be the case when ether - was the 
anesthetic used. Small amounts of blood withdrawn from time to 
time during such an experiment were found to in no way affect the 
percentage of normal chlorine in blood. The chlorine was estimated by 
the method of Carius. A known weight of blood, about three to five 
grammes, is treated with nitric acid and an excess of solid silver 
nitrate in a bomb tube heated to 200° C. for six hours. The amount of 
resulting silver chloride was then determined, and the difference oe 
= 
