6 BOTANICAL GAZETTE [JULY 
arated by so wide an interval that: the various accompanying 
changes may be readily seen even without the use of high mag- 
nifications. The successive steps in this process may be sum- 
marized as follows: Beginning with the immature ‘sporangial 
filament,” if we may use this term to indicate the structures from 
which or within which the spores are eventually produced, we 
find them filled as usual with undifferentiated granular protoplasm. 
The first indication of spore-formation is seen in the appearance 
of successive indentations of the protoplasm which correspond 
to the future lines of separation between the successive spores. 
These indentations extending completely around the sporangial 
filament thus divide it bya series of successive rings into a num- 
ber of segments corresponding to the number of spores to be 
produced. As this indentation gradually increases the proto- 
plasmic mass within the sporangial filament becomes correspond- 
ingly constricted, and by treating the specimen with eosin or 
other stains the indented area may be seen to be made up of two 
parts ( figs. 39-41), the one hyaline (az) unable to absorb stain 
and resembling in appearance the intersporal substance of ordi- 
nary sporangia, the other (4) acting toward stains like granular 
protoplasm. As the development proceeds the indentation just 
described and the corresponding constriction of the protoplasmic 
contents become more and more pronounced, while at the same 
time the stainable portion (fig. go.6) of what may conveniently 
be called the ‘“‘intermediary zone’’ increases in volume. At 
this stage the protoplasmic contents of the sporangial filament 
has become separated into distinctly formed oblong portions 
connected by gradually narrowing protoplasmic isthmuses (/g. 
41). These oblong portions become eventually completely sep- 
arated and are surrounded by a distinct wall which, however, on 
either side is hardly distinguishable from the wall of the spor- 
angial filament (fg. 42). In this mature condition the interme- 
diary zones may be seen as distinct rings (indicated by dotted 
lines in fig. 42), often distinctly elevated above the adjacent 
surface of the spores, probably by reason of the fact that they 
begin to become deliquescent almost as soon as the spore wall 
