1897 | BRIEFER AKTICLES 118 
sporocarps. Within three or four hours after sowing, several macro- 
spores were taken from each lot and passed separately under the 
microscope to insure that no microspores accompanied them. For 
this purpose a Leitz objective no. 3 and eyepiece no. 1 were used. 
The macrospores thus isolated were placed in distilled water in shal- 
low watch glasses and left standing beside the vessels containing the 
macrospores which were still mixed with microspores. 
The spermatozoids matured and were set free about eighteen 
liours after sowing, and when the specimens were examined twenty- 
one hours after sowing all the spermatozoids had been discharged. 
The archegonia on spores from the first and second sporocarps were 
counted at the end of one day, and the embryos in the same lots were 
counted and measured at the end of seven days. From the third and 
fourth sporocarps the embryos were counted at the end of four days. 
The following tables show the number of spores from which embryos 
were obtained, the number of spores which produced prothallia but 
no embryos, and the number which did not develop at all: 
SPORES FROM SPOROCARP I. 
With spermatozoids Isolated macrospores 
1 day 7 days 1 day 7 days 
Archegonia... .. 14 Embryos...... 20 | Archegonia.... 14 | Embryos....... 
Sterile yeerre'y Steri ee’ 
Sterile spore... .. I Sterile spores... 7 |Porenle spore... x Sterile spore... I 
15 36 15 15 
SPORES FROM SPOROCARP II. 
With sp | | Isolated macrospores 
t day 7 days 1 day 7 days 
Archegonia..... 5 | Embryos...... 22 || Archegonia ... 12 | Embryos....... 
Sterile oa. Sterile prothal- 
: Hahei s cuca 16 16 tee Sere 
Sterile spores.... 5! Sterile spores.. 27 |j Sterile spores.. 3, Sterile spores... 3 
10 65 15 15 
pe eee A: I : 
