116 BOTANICAL GAZETTE [AUGUST 
SPORES FROM SPOROCARP II], AFTER FOUR DAYS. 
Isolated macrospores 
With spermatozoids 
| 
| ’ Lota Lot 4 Lot ¢ Total 
Embryos Biss ode ae Ae | 2 3 5 10 
one prothallia ects rete coer 6 5 I 4 10 
LEME SHON CS 2 3 coe cues es eae | 3 6 I 10 
| —— 
aa 
35 | fe) 10 10 30 
SPORES FROM SPOROCARP IV, AFTER FOUR DAYS. 
Isolated macrospores 
With spermatozoids 
Lot a Lot & Total 
Embry Bs eetehets: Augen fet A Toe Per 19 4 2 6 
Sterile perenne ee ee ee I 4 6 To 
OPA IER kg pxdsc8 iKcy wed ea hao eke II 3 2 5 
ar} II 10 5 
Comparing the prothallia that produced embryos with those that 
were sterile we find 
Embryos Sterile Total Per cent. fertile 
sown with microspores . 69 32 101 69 
Isolated from microspores. . 33 29 62 53 
Briefly stated, over 50 per cent. of the isolated female prothallia 
produced embryos, while not more than 69 per cent. of those which 
were mixed with male prothallia produced embryos. 
In both cases, at the end of seven days the embryos were of three 
sorts: (1) those with the root and the cotyledon about equal in 
length; (2) those with the root less than one-third the length of the 
cotyledon ; and (3) those with no root developed. ‘Those of the first 
sort from the isolated spores were 1ro-12™" long, while those of the 
same sort from the mixed spores were 8-13"" long. Those of the sec- 
ond sort from the isolated and the mixed spores were 4-9" and 7-9"", 
respectively. The embryos of the third sort were 3-7" long from the 
