86 BOTANICAL GAZETTE [auGusT 
resulted from these cultures, which were made early in October, 
and they were discarded. On October 28 a Gloeosporium was 
found on the leaves of a sycamore on Ithaca Flats. The veins 
of the leaves had the withered appearance and were bordered by 
the irregular patches which characterized the disease on the oak. 
Upon the under side of the leaf brown acervuli were produced, 
on which an abundance of conidia were found. The acervuli 
were not confined so closely to the region bordering the veins as 
was the case on oak leaves, and the withered portions were more 
expanded, but the color of the pustules, and the spore mieasure- 
ments were the same, and the same peculiarity in germination 
was noted, the germ tube frequently coiling once and a half or 
twice around the conidium. The fungus was less contaminated 
on the sycamore, and the growth could be more satisfactorily 
observed. On the day following the sowing of the conidia 
many were found to be germinating, sending out one OF two 
germ tubes. On the third day after germination from three to 
five tubes could be seen to issue from a conidium which had in 
some cases become once septate, and the cells were slightly 
swollen. 
The colonies in nutrient agar produce a rather scant mycelial 
growth, the clusters of conidia forming grayish masses for the 
most part submerged in the agar, are borne quite uniformly ove? 
nearly the entire colony. The colony has a loose, feathery; 
almost uniform growth, there being no marked radiations of 
abundant central growth. In both meat- and potato-agar the 
growth is slow. On bean stems, also, there is a very slight, 
inconspicuous growth of mycelium, and the tube cultures at 
marked by the pale brown acervuli which are produced quite 
freely on the stems. 
ae June (1896), material was again we 
: oak leaves; the appearance on the leaf an 
section, as well as the peculiarity of germination and the gro' 
oe 
€ same species of Gloeosporium infests 
sycamore and oak. 
