1898 ] THE DEVELOPMENT OF SOME ANTHRACNOSES Iol 
genum no perithecial form was found in connection with it by 
the writer. In the study of G. nerviseguum (Fckl.) Sacc., both 
pycnidial and perithecial forms were found associated with it in 
the first dilution cultures, but when pure cultures were obtained 
by subsequent dilutions only the conidial form was found in the 
cultures. 
It is an interesting and significant fact that two species of 
Gleeosporium should be found associated with the same asciger- 
ous genus Gnomoniella. The writer made several attempts to 
obtain a culture of G. 7767s, all of which failed and its culture 
was abandoned. 
Gnomoniopsis cingulata Stoneman ( figs. 27, 28, 90-97); Gle@ospo- 
rium cingulatum Atk. on Ligustrum vulgare. 
In 1892 Professor Atkinson’? described a new species of 
anthracnose of the privet (Ligustrum vulgare). The growth 
characters of the fungus in artificial cultures suggested the prob- 
able cycle of development of this Glceosporium. On cultures 
of sterilized bean stems the threads were associated into strands 
of compact tufts, several layers deep. Within these wefts were 
numerous black rotund perithecia-like bodies, white within and 
filled with rich protoplasmic contents, presaging, as the author 
suggested, a probable ascigerous stage. Subsequent to the pub- 
lication of this study, mature perithecia were obtained in pure 
cultures of this Gloeosporium, sections of which were mounted 
and preserved. The material studied was obtained from Penn 
Yan, N.Y. The discovery of the perfect stage in pure cultures 
rendered the investigations of Professor Atkinson of more value 
than any results which had been previously brought to bear on 
the subject. 
In February 1895, material was received from Manhattan, 
Kansas, through Professor Hitchcock, of the State Agricultural 
College. The affected stems showed the elongated depressed 
areas of a light brown color corresponding to the affected twigs 
originally described. No spores could be obtained owing to the 
7 Cornell University Exp. Sta. Bull. 49: 310. 1892. 
