102 BOTANICAL GAZETTE [ AUGUST 
age of the fruiting pustules, but with a flamed scalpel, after cut- 
ting away the surface tissues, portions of the affected areas were 
removed and transferred to tubes of sterilized bean stems on — 
February 18. A grayish mycelium soon began to spread over 
the stems, and black perithecia-like bodies made their appear- 
ance in connection with the acervuli producing the masses of 
conidia. On March 18 an examination of the cultures was made, 
and the black elevated wefts of mycelium were found to be peri- 
thecia containing mature asci, agreeing in every respect with © 
those previously mounted by Professor Atkinson. 
The perithecia were cespitose, seated upon a subiculum or 
stroma of loosely interwoven mycelium, dark brown, flask-shaped, 
membranaceous, measuring from 250—320y in length and about 
150 in diameter, gradually constricted toward the apex into a 
short rostrum. The perithecia were more or less hairy with a — 
conspicuous tuft of coarse brown mycelium about the ostiolum. 
The asci were aparaphysate, clavate, sessile, measuring about — 
64 X 14m. Spores eight, hyaline, elliptical, slightly curved, 
subdistichous, 20-28 X 5-7, usually with a clear spot at the 
center. 
As it was impossible to say from what growth this result was 
obtained, it now remained to establish definitely the connection — 
of the ascigerous stage and the conidial form. A dilution cul- 
ture was therefore made in nutrient agar, and spores were — 
marked which by their size and shape could be distinctly recogy 
nized as ascospores. These spores germinated in the same : 
manner as do the conidia, by sending out a germ tube usually — 
near the end, a little in advance of one originating neat the 
opposite end. Fig. 97 shows germinating ascospores eight hours 
after sowing; twenty-four hours later under favorable conditions © 
they may attain a length of 500 or 600p, showing indefinite S€P” 
tation and irregular branching. The germination of some spores 
may be retarded and the germ tube delayed until the day fol | 
lowing the sowing, although the spores do not become altered 
in size or shape. On the second day after germination as many 
as four or five germ tubes may be seen to proceed froma single 
