1898 ] THE DEVELOPMENT OF SOME ANTHRACNOSES 103 
spore. These usually become branched quite near their origin, 
but the center of the colony remains open and the radiating 
mycelial strands remain distinct from each other for about 2™™ 
from the center. Beyond this the mycelium branches in a brush- 
like manner, mingling toward the margin in a loosely spreading 
uneven fringe. The colonies produced from the ascospore had 
the same characteristic snowflake appearance described by Pro- 
fessor Atkinson for the colonies resulting from the conidia. 
From the tips of the mycelium the elliptical or clavate conidia 
are delimited as early as the second day after germination; the 
time, however, varies, depending upon the separation of the 
colonies and the amount of nutriment. When the colonies are 
well separated and growing in an abundance of nutriment, the 
formation of conidia is delayed. In artificial cultures the 
acervuli are sometimes attended with setz, although they are 
not sufficiently abundant to characterize the genus as a Colleto- 
trichum, and none have thus far been found in sections of the 
acervuli made from the host plant. Very little pigment is 
developed in the agar in connection with the formation of 
conidia, but ten or fourteen days after sowing the colony begins 
to show a development of stromata in small circular masses 
scattered indefinitely over the colony, which become elevated 
above the agar and overspread it with a grayish mycelium. 
These are filled with coarse granular protoplasm, and represent 
the early stage of the perithecia ; the agar, however, usually 
dries away before they become mature, although asci have occa- 
sionally been found in them. These are formed in connection 
With conidia, alike on the colonies developed from either asco- 
Spores or conidia. Portions of the mycelium produced from 
marked spores were removed to cultures of bean stems, the col- 
Onies being sufficiently separated so as to insure pure cultures. 
These separation cultures produced the grayish mycelium, bearing 
conidia abundantly, which collect in pink masses. The mycelium 
after about ten days becomes a dark brown; numerous dark 
buds or gemmae (?) are formed, and the association of the 
Perithecia-producing stroma becomes manifest within a week or 
