1898 | THE DEVELOPMENT OF SOME ANTHRACNOSES 105 
condition varies in different cultures; in tubes containing richer 
nutrient material there is a more abundant and more compact 
development of mycelium. This difference is quite marked in 
parallel cultures on bean stems and young bean pods. In the 
latter case the mycelium is very abundantly developed, quite or 
nearly concealing the fruiting pustules. In tube cultures made 
on October 23 pink masses of conidia made their appearance five 
days later. In connection with the pink acervuli many perithe- 
cia-like bodies were observed. From this series of separation 
cultures a second dilution culture was made. The colonies again 
showed the nearly undifferentiated mycelial growth with the 
exception of a less abundant growth about the center. This 
portion, which is nearly free from mycelium at first, becomes 
overrun as the colony advances in growth, but the colony always 
remains more open at this portion. The production of conidia 
was not delayed so late in the second series of dilution cultures, 
and a few were formed eight days after sowing. Whether their 
earlier appearance was due to the condition of the nutrient agar, 
or to the fact that the fungus was becoming adapted to artificial 
growth conditions was not ascertained. The aggregated conidia 
form pale acervuli scattered quite thickly over the colony with 
the exception of the lighter portion near the center. In cultures 
ten or twelve days old the conidia become much longer than 
normal, and once or twice septate. From these cultures in which 
the colonies were so separated that pure separation cultures could 
be with certainty obtained, transfers were made on November 18 
to an infusion of bean stems. On December 15 an examination 
of the tube revealed fully developed perithecia in connection 
with conidial clusters and the colored club-shaped bodies. The 
perithecia were so covered by the mycelium that their presence 
was not detected until a portion of the growth was removed with 
a needle and examined with the microscope. In order to check 
the experiment a second sowing was made of conidia which 
had undergone desiccation in the laboratory for five months. 
These germinated and conidia and perithecia were obtained as 
re; 
befo 
