1898 | THE DEVELOPMENT OF SOME ANTHRACNOSES 109 
time unpublished, on Rudus strigosus, accompanied by the follow- 
ing description: “ Forming large, dark brown patches on the 
upper surface of the leaf; sori small, dark, suberumpent ; conidia 
oblong, elliptical, 12.5 x 6. Col. W. Va., Oct. ’95, F. W 
Nuttall.” 
Conidia sown in a dilution culture of potato-agar on January 
30, four hours later had developed germ tubes usually from one 
end of the conidium, and slightly constricted at the base, pro- 
ceeding in irregularly flexous manner, occasionally septate, with 
little or no branching for a distance of 100-150#. On January 
16 the growth could be seen in the agar with the unaided eye, 
the low temperature of the laboratory possibly accounting for 
the slow progress of the fungus. The mycelium showed a long, 
narrow, loosely branched growth, 3-4™™ in length. A colony 
from one of the conidia, which had been marked at the time of 
germination, was transferred to sterilized bean stems. Four 
days later the mycelium had formed a rather dense, closely 
adhering weft of grayish mycelium; black fruiting bodies 
appeared upon the stems, which were also overspread with a 
mycelial growth. Upon examining these on February 10, the 
perithecia were found to contain mature asci, the ascigerous 
Stage agreeing generally in appearance with the two previous 
forms. There is lacking in this species the conspicuous tuft of 
mycelium at the apex of the perithecia, which are usually larger 
than those of the pepper anthracnose. 
‘The ascospores germinated in potato-agar, as had the Col- 
letotrichum conidia, by sending out obliquely from one end a 
germ tube ; the tube again is slightly constricted at the base, 
and extends from 180-200, in length before branching takes 
Place. This growth produces a colony, which, like that from 
the Colletotrichum conidia, is at first narrow, elongate, and 
loosely Spreading. About four days after germination, conidia 
are delimited from the mycelium in great abundance. Later, 
colored swollen buds are formed. The reproduction of conidia 
here took place much sooner than in the original cultures of 
the conidia ; and, inasmuch as the temperature of the laboratory 
