170 BOTANICAL GAZETTE [ SEPTEMBER 
detached leaves of Funaria hygrometrica, but no buds were pro- 
duced. He also makes the very broad statement: ‘‘Chaque 
feuille et méme chaque portion de feuille detachée de la plante- 
mere et placée dans les cgnditions convenables peut produire des 
filaments proembryonnaires, par la multiplication d’une ou de 
pleuiseurs de ses cellules parenchymatuses.”’ Goebel’ also 
mentions the ability of Funaria leaves to produce protonemata, 
when they are detached and kept moist. Limpricht® states that 
almost every leaf can by proper culture be made to form sec- 
ondary protonemata. Also in the case of plants with brittle 
leaves, as Leucobryum glaucum, Barbula fragilis, Campylopus frag- 
tis, and. Barbula ruralis, one can find in nature on the detached 
leaves the beginnings of protonemal filaments. 
It is to be noted that in all of the cases above mentioned, 
regeneration only occurred when the leaves were detached from 
the stem. That this is not necessary in all cases is shown by 
the observations of Goebel? on the leaves of several species. 
In Oncophorus glaucus a thick felt of tangled filaments appears on 
the fertile summits of the plants, which prevents their further 
growth and eventually gives rise to patches of young plants. 
The marginal cells of Buxbaumia aphylla \eaves are able to pro- 
duce protonemata which will completely envelop the leaf. 
According to Limpricht,® the apex of the end bud in Leucobryum 
has been known to produce a protonemal growth, and H. Schulze 
has observed a luxuriant growth of protonemata from the leaf 
apices of Hypnum giganteum. 
Mention should be made here of the formation of brood- 
bodies on different portions of the leaf, now apex, now costa, in 
various species of Orthotrichum, Ulota, Barbula, Grimmia, 
Syrrhopodon, and Calymperes.2 These brood-bodies are appat- 
ently formed in the younger stages of the leaf and are homol- 
5 Sitz.-Ber. d. mat.-phys. Classe d. k. bayr. Akad. d. Wiss. 26: 463. 1896. 
® Laubmoose von Deutschland 1: 64. 
7 Outlines of Classification 173. 1887. 
8 Loc. cit, 
9GOEBEL, Outlines of Classification 172-173. 1887. LIMPRICHT, Laubmoose 
4. 
