172 BOTANICAL GAZETTE [SEPTEMBER 
and Bryum cespiticium, all in artificial cultures, and by Brizi,” in 
nature for Funaria hygrometrica. According to Brizi, some of 
the setae of Funaria which had come into contact with the earth 
produced an abundant growth of protonemata with numerous 
buds. 
Ill. METHOD. 
In course of the experiments described below three different 
methods were used. The leaves and stems to be used as cultures 
were carefully washed in sterilized water in order to render them 
as free as possible from bacteria and fungi, and then placed 
either in Petri dishes upon several thicknesses of filter paper 
which had been saturated with a nutritive solution, or upon 
pieces of flowerpots placed in crystallizing dishes. In the third 
method the leaves were placed upon soil in either Petri or crys- 
tallizing dishes. The filter paper was carefully sterilized in boil- 
ing water and then placed in the Petri dishes which had been 
previously sterilized in the dry-oven. The pieces of flowerpots 
were first boiled and then sterilized together with the crystalliz- 
ing dishes in the dry-oven. The dishes containing the soil were 
also sterilized in the same way. All of the cultures were sup- 
‘plied with a 4% pro mille normal nutritive solution, and were 
kept at a temperature varying between 19—21° C. 
IV. EXPERIMENTAL. 
In course of my investigations the following species were 
used: Mnium rostratum Schwagr.; Funaria hygrometrica Hedw.; 
Bryum capillare Hedw.; Bryum argenteum Linn.; Barbula murals 
Timm.; Awichum undulatim P. Beauv.; Polytrichum commune 
Linn.; Brachythecium rutabulum Bry. Eu. and variety ; Leptobryum 
pyriforme Schimper; Phascum cuspidatum Schreb.; Ceratodon 
purpureus Brid.; Fissidens bryoides Hedw. 
In addition to these, cultures of Plagiochila asplenoides and 
Lophocolea bidentata were made for comparison with those 9 
Schostakowitsch. 
*® Annuar. Istituto Orto botan, Roma 5 : 53-57. 1892. 
