234 BOTANICAL GAZETTE [OCTOBER 
IV. TELOPHASE. 
7. The chromosomes having oriented themselves around the 
poles, now begin to contract, becoming wavy in outline, and 
the free ends curve inward. The threads of the central spindle 
begin to thicken preparatory to the formation of the cell plate. 
In the center of each thickened thread a granule appears, these 
being formed first in the central strands, and as the spindle 
bulges outward the cell plate gradually enlarges until it reaches 
the surrounding cell wall. In the meantime the nucleoli begin 
to appear in the daughter nuclei. This stage may be called the 
loose daughter skein, and may be considered to end when the cell 
plate is complete (figs. 34-39). 
8. After the daughter cells are completely separated by the 
new cell wall the threads of the central spindle disappear, and 
the daughter nuclei appear with complete nuclear membranes. 
The chromosomes begin to be transformed again into the chro- 
matin network; the radiations disappear from around the cen- 
trospheres, which have now usually divided completely into two 
separate bodies; and the two daughter nuclei in the meantime 
expand and take on amore spherical form until they enter again 
into the resting stage (figs. go-g2). This stage may be known 
as the close daughter skein. 
_4ig. 1 may be taken as a typical nucleus in the tissue beyond the growing 
point, showing in detail the actual arrangement of the chromatin network, 
chromatin granules, and nucleolus, fig. 2 represents a typical cell in the 
active part of the meristematic region. 
To illustrate the normal order of karyokinesis, the following figures may 
be taken as a complete series: 2, 4: 5,8s11, 13, 16,17; 20, 22,25; 20,27; 
29,391 321 33 * 34,3536,38 ; 39, 40,42. A briefer series may be represented 
by the following: 2, 5:8, 13,27, 20, 25, 20, 27, 70, 72, 33, 74s FOr 39s $s 
SAGITTARIA VARIABILIS. 
The anilin-safranin, iron-alum-haematoxylin combination was 
also tried on dividing poilen mother cells of Sagittaria. The 
results were even more striking than in the onion. Inthe mother 
star stage the centrosomes at the poles look like large black 
