242 BOTANICAL GAZETTE [OCTOBER 
F’. Rosen3 describes spindles quite similar to the above in 
the root tips of Hyacinthus orientalis. He finds the spindle aris- 
ing as acap-shaped prominence which is formed on two opposite 
points of the nucleus by the concentration of “kinoplasm” 
which had formed a hyaline area around the nucleus. This 
spindle originates before the dissolution of the nuclear mem- 
brane. 
I examined carefully a large amount of material in search 
for radiations around the nuclei and for multipolar spindles in 
the prophases of karyokinesis as figured by Osterhout,‘ Mottier,' 
Juel,° and Debski,” but I was unable to find a single cell in these 
early stages that showed such structures. However, as above 
observed, in some preparations strands of cytoplasm were seen 
around the nuclei of nearly every cell. This was observed in 
cells both in the resting stage and during karyokinesis, These 
radiations were very coarse and could not, I think, be instru- 
mental in spindle formation. 
In some injured or sliced karyokinetic figures I found, 
though very rarely, spindles which appeared to be multipolar. 
The cells containing such spindles were all in the anaphase, 
mainly in the metakinesis and mother star stages (fig. 27), at 
which time the spindle is elongated and is more likely to be 
sliced in sectioning than those which are in other stages of 
karyokinesis. 
In the material examined many cells were observed in the 
prophase, a large number of which showed definite spindles. 
In every case the spindle was bipolar, being short and rounded, 
or dome-shaped, when first visible near the nuclear membrane. 
The evidence furnished by my investigation is opposed to the 
theory that the spindle of Pinus originates as a multipolar 
structure. 
With the segmenting of the spirem the metaphase begins. 
The spirem is scattered throughout the nucleus in the outer 
3 COoHN’s Beitrage zur Biologie der Pflanzen 7 : 225~312. 1895. 
4 Jahrbiicher fur wiss. Bot. 30: 159-168. 1897. 6 Ibid. 30 : 205-226. 1897: 
* Ibid. 30: 169-204. 1897. 7 Ibid, 30 : 227-248. 1897- 
