1909] STEVENS & HALL—VARIATION OF FUNGI 3 
SEPTORIA LYCOPERSICI SPEG., FROM TOMATO 
Spores from pure culture were plated in 4 per cent. pea agar in 
various dilutions. 
One plate developed 5 to 6 colonies per square millimeter and each 
colony proceeded to normal pycnidial development. Another plate 
developed 21 to 23 colonies per square millimeter, and all proceeded 
to form naked conidia with no indication of pycnidia. Portions of 
these two plates are represented by photomicrographs (jigs. 1, 2). 
Drawings of the naked spores showing the detail of their formation are 
given in fig. 3. Occasionally plates with as many as 30 colonies per 
square millimeter were found with both pycnidia and naked spores. 
Pycnidia not visible at the fifth day may be well formed by the 
eighth day and extrude masses of pink spores about the twenty-first 
day. Occasionally pycnidia 
are well developed on the 
fourth day. When naked 
spores develop they normally 
appear a few days later than 
do pycnidia, e. g., a plate 
thinly sown January 12, 1907, 
gave many pycnidia on Jan- 
uary I5; while a thickly sown Fic. 3.—Mode of formation of naked 
plate, under conditions other: “Po under influence of crowded culture. 
wise precisely parallel, did not give naked spores until January 22. 
This Septoria forms a typical determinate colony, i. e., even with un- 
limited room, it proceeds only to a certain size of development. 
SEPTORIA CONSIMILIS E. & M., FROM LETTUCE 
When sown thinly, colonies reached a size of 2 to 3™™ in diameter; 
when sown thickly, they became no more than o.2™™ in diameter. 
There was no interference with color development or formation of 
pycnidia by thick sowing with this species. 
With two of these septorias, thick plating, other conditions being 
the same, so changed their character that not only would the species 
be considered as different, but the fungus would be shifted from the 
order Sphaeropsidales to the order Hyphomycetales (Hyplaesa of 
SACCARDO). 
