Io BOTANICAL GAZETTE [JULY 
III. Chemical relations 
Chemical relations have been studied with eleven fungi, the fungus 
being usually grown in agar with varying nutrients added. Occa- 
sionally other media were used. A chemical base agar (CBA) was 
1G. 9.— Sclerotinia Libertiana Fuck. jcoteds the formation of sclerotia in greater 
abundance where adjacent colonies come in contac 
made of the following proportion (grams); water 1000, di-potassium 
phosphate 2.5, magnesium sulfate .or, calcium chlorid .or, sodium 
chlorid 2.5, potassium sulfate 2, agarrs. To 100°¢ of this chemical 
base agar were added the following materials (grams) singly or in 
varying combinations: ammonium lactate o. 5, sodium asparaginate 
0.25, glucose 1, starch 1. The tests were usually made in both plate 
and tube cultures. 
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