14 THE BOTANICAL GAZETTE [JULY 
Spore formation proceeded sparingly, though evenly and regularly, 
upon pure agar, CBA, CBA+ammonium lactate, CBA+sodium 
asparaginate, CBA+sodium asparaginate-+ammonium lactate; but 
very abundantly upon CBA+ sodium asparaginate+ starch and upon 
CBA+sodium asparaginate+glucose. Here the sodium asparag)- 
nate seems not to furnish the carbon in sufficiently available form, 
though starch or glucose do so to nearly equal extent. 
The size, color, and septation of the spores were also greatly influ- 
enced by the medium. From carnation-agar plates the spores 
measured 16 to 52 # long by 6 to 13 # thick, bearing 0 to 3 longitudinal — 
septa and three to seven transverse septa; while from the live carnation 
leaf the spores were 26 to 123 # long by 10 to 20 thick, bearing 1 t09 © 
or often numerous longitudinal septa and 3 to 15 transverse septa. 
It is seen that the spores are approximately twice as long, twice as 
thick, of darker color, and with many more septa in each direction 
upon the natural medium than upon the carnation agar, differences 
which would ordinarily be regarded as clearly of specific rank. 
ALTERNARIA BRASSICAE (BERK.) SACC., FROM COLLARD 
This fungus made hyaline mycelium in CBA and CBA+sodium 
asparaginate; black mycelium in CBA-+sodium asparaginate+ 
glucose and in CBA+sodium asparaginate-+starch, starch producing 
by far the most pronounced effect. 
Digestion of the starch grains, somewhat in advance of the tips 
of the oncoming fungous threads, produced a clear zone surrounding 4 
each colony in the starch-bearing plates. 
ASCOCHYTA CHRYSANTHEMI STEVENS 
This fungus was grown in the usual media with no significant 
effects, except that the fungus did not digest the starch grains afforded 4 
in the medium. 
A deposit of great thickness around mycelial threads was made in 
the case of certain media and not in others, as has already been 
noted.'° 
In some instances culture at a high temperature occasioned this 
same reponse. 
0 Bor. GAZETTE 44: 241. 1907. 
Pa ite ary 
