1909] OTTLEY—JUNIPERUS 37 
ing spring. In my material, the macrospore mother cell in J. communis 
was never differentiated until the spring following pollination. 
Soon after pollination, May 11 for J. communis, the ovules cease 
to grow and remain practically the same size until the following spring 
(figs. 15, 16). In J. virginiana, however, the ovules continue to 
grow, the macrospore mother cell is differentiated, the female game- 
tophyte is formed, and fertilization takes place in the latter part of 
June or the first of July of the same year in which the ovules were 
pollinated. StupskKy (22) described fertilization in J. communis 
as taking place in the same year as pollination. Both Norg&n’s 
studies and the present investigation show that over a year elapses 
between pollination and fertilization in J. communis. It seems highly 
improbable that Stupsky could make a mistake in the age of the 
cones with which he was working, as suggested by CHAMBERLAIN 
(3) ina recent review in the BoTaNICAL GAZETTE. Since the present 
study shows conclusively that the time elapsing between pollination 
and fertilization in different species of Juniperus may vary by nearly 
a whole year it seems far more probable that SLUDSKY was working 
with some other species than J. communis, and that in the species 
with which he worked fertilization does take place in the summer 
following pollination, as is the case in J. virginiana. 
In J. communis growth began again in the early part of April. 
The macrospore mother cell appeared April 14 in the basal portion 
of the nucellus, and three days later the cell divided. In the prophase 
of the first division the cytoplasm is vacuolate except at one point, 
a short distance below the nucleus, where it is dense and stains more 
deeply (jig. 18), as noted by Norén. A similar densely staining 
mass of cytoplasm is present in all stages of division in the macrospore 
mother cell. No connection between this body and the division figures. 
could be traced. CoKER (5, 6) observed a similar condensation in 
Taxodium and in Thuja, but was unable to determine its significance. 
The presence of synapsis, the character of the spirem after synapsis, 
and the shape of the chromosomes indicate that this is a heterotypic 
division. In one slide studied there seemed to be evidence of a four- 
celled axial row, but from the material examined it was impossible 
_ to determine definitely whether the axial row consists of three or four 
cells. Norén states that there are three cells. 
