"om BOTANICAL GAZETTE ~ [yowy 
sporocytes. I have previously recorded the occurrence of extra, imperfect — 
sacs in Castalia, Brasenia, and Cabomba, but in all cases I considered them | 
to have been derived from independent archesporial cells. In this new — 
case, to which I now refer, it was evident that the second sac had not been 
penetrated by a pollen tube, and of course the endosperm had been formed 
without triple fusion. In this connection I wish to say that I have previously . 
observed sacs of species of this family which were filled with typical endo- ~ 
sperm, but without embryos, and in which it appeared that there hate 
been no fertilization. . 
However, in the normal embryo sacs of Nelumbo lutea, the polar nude . 
united in the usual manner and then divided to form the endosperm. " 
was unable to follow the early divisions of the endosperm, but in a few © 
first division of the endosperm (fig. 10). The tube nucleus (f) was ¢ 
paratively small and undergoing disintegration. The antipodal end 
the sac is in contact with an axial mass of cells (fig. rr), which may consist 4 | 
of a single row of cells or many rows. These cells are rich in protoplasm 
and eventually disintegrate, probably forming food for the develop 
embryo. They have been referred to by York 
very similar to the cells in the secondary sac (fig. 8), and I am inchne¢ 
to consider them as the vestiges of undeveloped embryo sacs. Near 
cross-wall, but in the micropylar chamber of the sac shown in fig. 10, 
a rather compact mass of cells (d), which somewhat resembles a seco! 
embryo,” but a careful examination leads me to believe it a part of 
endosperm. 
In one ovule of Castalia odorata the embryo was well advanced, althoug 
the endosperm nucleus had remained undivided (fig. 13). 
EMBRYOS 
In my first paper I stated that the embryos are without suspensdl 
