ea ee ee eee Bn ae eee 
1909] LIPMAN—EFFECTS OF SALTS ON BACILLUS 113 
solutions have a uniform bulk of 100°, thus avoiding any differences 
in the supply of oxygen to the bacteria by having an equal surface 
of liquid exposed to the air in each flask. 
As in the case of the single salt solutions, duplicates were run on 
all the cultures, and also sterile controls, so as to allow of a deter- 
mination of the ammonia actually formed by the bacteria. The 
solutions were all sterilized in the autoclave at 1.25 atmospheres, 
inoculated as above, and incubated for two and a half days at 28° to 
29° C., after which they were distilled, as in the case of the single salt 
cultures, and the ammonia determined. The results obtained follow, 
with the curve plotted from them in accordance with the arrange- 
E : : 
ALL QUANTITIES GIVEN REFER TO CUBIC CENTIMETERS OF 0.35 ## SOLUTIONS 
Culture solution Lip yeni per _ 
Heb KC es ss K ores 
es CaCl, : i 21.46 
mi ack, . Nee EER if 18.83 
ee CaCl, : H 9-00 
"So CaCl, § G 6.44 
se CaCl F 6.30 
too Ot: Pepa E 4-55 
100 Gch. arieeipae Sate D 0.25 
ns eee : cC 0.14 
o Be ; seus GAS B 0.25 
WOO Cai gear yn A 0.00 
ment employed by OsrerHouT (19). The letters along the axis of 
abscissas represent a given combination of the two salts as indicated 
in the table, and the ammonia formed is laid off on the axis of ordinates 
in numbers representing milligrams. 
We see at a glance (fig. 2) that there is a strong antagonism 
